Extracellular Vesicle Isolation and Analysis by Western Blotting.
نویسندگان
چکیده
Extracellular vesicles (EVs) are released by mammalian cells and are thought to be important mediators of intercellular communication. There are many methods for isolating EVs from cell culture media, but the most popular methods involve purification based on ultracentrifugation . Here, we provide a detailed protocol for isolating EVs by differential ultracentrifugation and analyzing EV proteins (such as the tetraspanins CD9 , CD63 and CD81 ) by western blotting.
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ورودعنوان ژورنال:
- Methods in molecular biology
دوره 1660 شماره
صفحات -
تاریخ انتشار 2017